FastQCFastQC Report
Thu 6 Feb 2020
H5VJNBGXF_n01_Salt_NSF_Plate_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJNBGXF_n01_Salt_NSF_Plate_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365014843
Sequences flagged as poor quality0
Sequence length20
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG117980483.2322104775339233No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTAC4840550.013.880224
CTTCGTC8027750.013.8770172
TCAACCG3669750.013.8588431
TCCTTAC4012900.013.8464244
CCACACT3585150.013.84324552
CCTCTCT2747150.013.8428864
TCTCCTA4853400.013.84015753
CCTACGT4847950.013.8389086
TCCTACG4854500.013.8347495
TCGACCA3636500.013.8264062
AATCCTT3990600.013.821132
GTCAGCT8025550.013.8148196
CCTTACG4026950.013.8115985
TCGACTT3374300.013.8069746
TTCGTCA8063350.013.8041713
GTCTCCT4875050.013.7987722
AACTACC3292150.013.7970586
AACCGAT3721350.013.7943213
AGGACGA7198600.013.789193
GTCCTCT2836700.013.786282
Produced by FastQC (version 0.11.5)