FastQCFastQC Report
Thu 4 Apr 2019
H5VJGBGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJGBGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37873269
Sequences flagged as poor quality0
Sequence length99
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG613292116.19327077364249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG583050.060.4148982
AAGCAGT694450.051.3831671
AGTGGTA768350.046.4289675
GCAGTGG788100.045.5812263
GTATCAA746350.045.4949729
GGTATCA788000.044.0205848
CAGTGGT816250.043.233614
GTGGTAT846200.041.9086346
TGGTATC903150.038.4221537
AGTACAT710800.027.3512320-21
TACATGG704350.027.15898722-23
AGAGTAC757550.026.4069818-19
ATCAACG736150.026.23161310-11
CAACGCA742700.025.81642712-13
CATGGGG496600.025.4562824-25
GTACATG680650.025.19786522-23
GCAGAGT823900.024.41506416-17
CGCAGAG747650.023.86067416-17
CAGAGTA768800.023.20801718-19
ACATGGG732950.023.07497624-25
Produced by FastQC (version 0.11.5)