FastQCFastQC Report
Thu 4 Apr 2019
H5VJGBGXB_n02_ICAL1-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJGBGXB_n02_ICAL1-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41862451
Sequences flagged as poor quality0
Sequence length99
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG890060.21261535785374822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1013200.058.0339472
AAGCAGT1105950.053.5161631
AGTGGTA1105050.053.4486585
GCAGTGG1141300.051.6590083
GTGGTAT1264550.046.6500636
CAGTGGT1268550.046.3990864
GGTATCA1273200.045.9145938
TGGTATC1339700.043.7385987
GTATCAA1320200.043.7247479
ACGCAGA1138250.028.46227514-15
CAACGCA1179300.027.48016212-13
AGTACAT1203200.026.90596620-21
TACATGG1210000.026.40654222-23
GCAGAGT1270000.025.8945816-17
ATCAACG1300050.025.26511410-11
CATGGGG804500.025.18970924-25
AGAGTAC1312750.025.07702618-19
GTACATG1149350.024.75560222-23
TATCAAC1212700.023.7724110-11
TCAACGC1211650.023.54609912-13
Produced by FastQC (version 0.11.5)