FastQCFastQC Report
Thu 4 Apr 2019
H5VJGBGXB_n02_ICAL1-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJGBGXB_n02_ICAL1-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52205078
Sequences flagged as poor quality0
Sequence length99
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1162770.22273120633973575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1388400.058.671532
AAGCAGT1490400.054.8704071
AGTGGTA1507150.054.1592455
GCAGTGG1560950.052.1971933
GTGGTAT1722150.047.4932336
CAGTGGT1737150.046.9587864
GGTATCA1743300.046.695948
GTATCAA1793500.044.9394729
TGGTATC1830600.044.604817
ACGCAGA1581250.028.8337114-15
CAACGCA1635200.027.92493612-13
AGTACAT1663200.027.36941120-21
TACATGG1680950.026.7658822-23
GCAGAGT1725550.026.67918616-17
AGAGTAC1795050.025.62748118-19
ATCAACG1798600.025.61616510-11
CATGGGG1131750.025.57682424-25
GTACATG1593150.024.97061322-23
TATCAAC1674800.024.11591710-11
CGCAGAG1699750.023.80685216-17
Produced by FastQC (version 0.11.5)