FastQCFastQC Report
Thu 4 Apr 2019
H5VJGBGXB_n02_ICAL1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJGBGXB_n02_ICAL1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52919543
Sequences flagged as poor quality0
Sequence length99
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1201000.2269482939412383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1387550.058.6622852
AAGCAGT1493750.054.9023671
AGTGGTA1504600.053.953455
GCAGTGG1553800.052.28053
GTGGTAT1716650.047.3273166
CAGTGGT1747100.046.5277984
GGTATCA1746200.046.2600488
GTATCAA1796700.044.565139
TGGTATC1826050.044.3583347
ACGCAGA1580550.028.7302514-15
CAACGCA1631800.027.85344712-13
AGTACAT1660600.027.33104720-21
TACATGG1677450.026.62644822-23
GCAGAGT1723250.026.62164316-17
AGAGTAC1788300.025.698418-19
ATCAACG1802200.025.43097910-11
CATGGGG1114900.025.21921324-25
GTACATG1579050.025.02292822-23
TATCAAC1675500.023.96583710-11
CGCAGAG1702900.023.7108816-17
Produced by FastQC (version 0.11.5)