FastQCFastQC Report
Thu 4 Apr 2019
H5VJGBGXB_n02_ICAL1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJGBGXB_n02_ICAL1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34326388
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG789330.22994845831143088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG896000.058.8594062
AAGCAGT969150.054.7758371
AGTGGTA975950.053.9304735
GCAGTGG1007400.052.1570783
GTGGTAT1118250.047.0773436
CAGTGGT1134450.046.2944644
GGTATCA1132500.046.219068
TGGTATC1183000.044.449447
GTATCAA1172050.044.2444279
ACGCAGA1022850.028.80018614-15
CAACGCA1051350.027.9760112-13
AGTACAT1077200.027.33570520-21
TACATGG1086600.026.78449622-23
GCAGAGT1118100.026.56156716-17
AGAGTAC1158850.025.6544418-19
ATCAACG1160150.025.63635410-11
CATGGGG731050.025.52913724-25
GTACATG1028250.024.95348722-23
TATCAAC1086850.023.9366510-11
CGCAGAG1098300.023.75509316-17
Produced by FastQC (version 0.11.5)