FastQCFastQC Report
Thu 4 Apr 2019
H5VJGBGXB_n02_CTR1-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJGBGXB_n02_CTR1-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59243634
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1420980.23985361870272845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG2000150.065.4888842
AAGCAGT2058600.063.946071
AGTGGTA2384400.054.9760445
GCAGTGG2391300.054.9212883
CAGTGGT2571300.050.9264144
GTGGTAT2648600.049.3864636
GGTATCA2652200.048.923138
TGGTATC2745400.047.4272547
GTATCAA2724950.046.966639
ACGCAGA2505150.028.99765614-15
CAACGCA2540850.028.55914912-13
AGTACAT2609600.027.8629420-21
GCAGAGT2667700.027.65317516-17
TACATGG2624650.027.51555322-23
AGAGTAC2726250.026.99204418-19
CATGGGG1727300.026.85623424-25
ATCAACG2722500.026.79844310-11
GTACATG2532850.025.34422122-23
TCAACGC2579700.024.73705712-13
CGCAGAG2640050.024.72668816-17
Produced by FastQC (version 0.11.5)