FastQCFastQC Report
Thu 4 Apr 2019
H5VJGBGXB_n02_CTR1-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJGBGXB_n02_CTR1-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59773033
Sequences flagged as poor quality0
Sequence length99
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1537610.2572414218967273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG2014200.065.343842
AAGCAGT2078500.063.5794261
AGTGGTA2401050.054.8286935
GCAGTGG2407950.054.7632333
CAGTGGT2594800.050.6507574
GTGGTAT2678550.049.1173826
GGTATCA2683800.048.5538948
TGGTATC2781450.047.0768937
GTATCAA2749200.046.7477879
ACGCAGA2531250.028.76835814-15
CAACGCA2568400.028.32635912-13
AGTACAT2631200.027.64533220-21
GCAGAGT2694450.027.40687416-17
TACATGG2647000.027.29913922-23
AGAGTAC2745950.026.79859418-19
CATGGGG1757250.026.72323824-25
ATCAACG2751650.026.64467610-11
GTACATG2554200.025.06875422-23
TCAACGC2605050.024.55811712-13
TATCAAC2629450.024.4809110-11
Produced by FastQC (version 0.11.5)