FastQCFastQC Report
Thu 4 Apr 2019
H5VJGBGXB_n02_CTR1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJGBGXB_n02_CTR1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35613523
Sequences flagged as poor quality0
Sequence length99
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG971590.2728149079775118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1214150.065.342822
AAGCAGT1238150.064.223771
GCAGTGG1459000.054.7016833
AGTGGTA1458400.054.600065
CAGTGGT1567100.050.7086754
GTGGTAT1615250.049.1689226
GGTATCA1614300.048.6505178
TGGTATC1676950.047.089667
GTATCAA1649650.046.724469
ACGCAGA1521750.028.89016714-15
CAACGCA1535250.028.50723612-13
AGTACAT1577800.027.82977920-21
GCAGAGT1619100.027.68640716-17
TACATGG1588850.027.4678322-23
AGAGTAC1650400.027.07509418-19
CATGGGG1066300.026.99232924-25
ATCAACG1639850.026.88206310-11
GTACATG1530350.025.1618122-23
TCAACGC1556350.024.62694712-13
TATCAAC1571600.024.5482810-11
Produced by FastQC (version 0.11.5)