FastQCFastQC Report
Thu 4 Apr 2019
H5VJGBGXB_n02_CTR1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJGBGXB_n02_CTR1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52821768
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1223050.23154279879461814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1769850.065.196372
AAGCAGT1829500.063.4067231
GCAGTGG2122300.054.4124763
AGTGGTA2132050.054.2611435
CAGTGGT2294300.050.280794
GTGGTAT2373000.048.7661066
GGTATCA2386050.048.1499678
TGGTATC2464800.046.7424627
GTATCAA2463200.046.234089
ACGCAGA2252400.028.71863714-15
CAACGCA2285950.028.29344712-13
AGTACAT2353500.027.45240420-21
GCAGAGT2394750.027.2468216-17
TACATGG2351350.027.24084922-23
AGAGTAC2444550.026.67942218-19
ATCAACG2449700.026.58281710-11
CATGGGG1558600.026.50121324-25
GTACATG2277500.024.87992722-23
TCAACGC2317500.024.43640912-13
CGCAGAG2363900.024.29680316-17
Produced by FastQC (version 0.11.5)