FastQCFastQC Report
Thu 4 Apr 2019
H5VJGBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJGBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37873269
Sequences flagged as poor quality0
Sequence length27
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGG600876815.865459092004972No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGC7957702.1011389325806547No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGT1303710.3442295937010349No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTG582860.15389746261406692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGC932600.018.38001821
GACTAAC54800.018.0748561
GCGCGAT49550.017.806531
CGAACGG37850.017.363429
GGGAATG49850.017.3622381
GGGACCT42800.017.3262521
CCGGGAT51650.017.2655741
CTCGAAA54050.017.1596581
TGACTAG56050.017.147041
GACTGCG52200.017.1037791
CGCTTCA75850.017.0746231
GATCTAG70050.017.063881
AGGCGAA28550.017.06230210
GGAACTT81150.017.0078931
CTGATCC64200.016.966311
TCGAACG39050.016.9642498
GGAATAA66000.016.9173761
CGGCTAG88400.016.7655741
ATGCGAT46050.016.7649151
GCATGCG58100.016.7408891
Produced by FastQC (version 0.11.5)