FastQCFastQC Report
Thu 4 Apr 2019
H5VJGBGXB_n01_ICAL1-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJGBGXB_n01_ICAL1-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41862451
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGA205950.019.4320811
GCGGACA108600.019.1439343
ACTGATG161400.018.8864351
TGAGCCG120850.018.835121
TGCGGAC110700.018.714442
CGGACAG107100.018.4218224
TCCGCGA123500.018.39718410
GGGACCT118950.018.3942011
AGGATGG122900.018.2990538
AGTGAGG153250.018.1978551
TGCGTGG137900.018.098321
CCGGGAT129400.018.0776561
GGGCATC116600.018.0352291
ACGAGGA145550.018.0131021
GGGAAAC89400.017.9568849
AGGACGA63050.017.9012938
GAATGAA141400.017.8732011
CAATGGA98050.017.8397378
AAACCTG166000.017.7619421
AGCACCG161500.017.7610748
Produced by FastQC (version 0.11.5)