FastQCFastQC Report
Thu 4 Apr 2019
H5VJGBGXB_n01_ICAL1-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJGBGXB_n01_ICAL1-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52205078
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGA258050.019.3027671
GCGGACA133700.019.1859323
ACTGATG209950.018.8619821
TGAGCCG147100.018.5804621
TGCGGAC138250.018.509052
AGGATGG150650.018.4895948
TCCGCGA154900.018.42964710
GGGACCT148500.018.3557781
CGGACAG132300.018.3412844
CAATGGA113500.018.1124028
GGGCATC145600.018.0865121
AGGACGA77750.018.0682038
ACGAGGA177550.018.0561731
GAATGAA169550.018.0283971
TGCGTGG170050.017.94451
CCGGGAT164750.017.9415741
GGGAAAC116000.017.9302259
GACTAAC132350.017.9130481
AGTGAGG192200.017.9095611
AAACCTG206350.017.8675291
Produced by FastQC (version 0.11.5)