FastQCFastQC Report
Thu 4 Apr 2019
H5VJGBGXB_n01_ICAL1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJGBGXB_n01_ICAL1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52919543
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGA257350.019.4452861
GCGGACA136350.019.0672913
ACTGATG202750.018.8999541
TGCGGAC137800.018.7905142
TCCGCGA152550.018.78927210
TGAGCCG147350.018.7416231
AGGATGG152800.018.4836628
GGGACCT148100.018.3700981
TGCGTGG178750.018.0820731
CGGACAG133850.018.0662784
ACGAGGA185350.018.0105951
CTGATCC198600.017.9990461
AAACCTG209700.017.9679991
GATCTAG225050.017.9280091
CCGGGAT162250.017.9268931
AGGACGA81250.017.910148
ATAACGC144800.017.845691
AGTGAGG191150.017.8377881
GGGAAAC115100.017.833259
GAATGAA170450.017.8163131
Produced by FastQC (version 0.11.5)