FastQCFastQC Report
Thu 4 Apr 2019
H5VJGBGXB_n01_ICAL1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJGBGXB_n01_ICAL1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34326388
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGA166050.019.5211811
TGCGGAC91100.018.9023442
GCGGACA91050.018.8665963
ACTGATG133800.018.864561
TCCGCGA103300.018.70148310
AGGATGG99000.018.580538
TGAGCCG97300.018.5787731
GGGACCT95550.018.3144261
AGGACGA51800.018.2419788
TGCGTGG112100.018.1685891
CGGACAG88100.018.1634624
CCGGGAT107050.018.103341
GACTAAC92950.018.0921821
GGGCATC96100.017.9800761
GGGAAAC74700.017.9204449
AGTGAGG125350.017.8653661
ACGAGGA120450.017.8508971
GAATGAA112850.017.8244551
ACGAGCC93400.017.8025861
AAACCTG133650.017.8011591
Produced by FastQC (version 0.11.5)