FastQCFastQC Report
Thu 4 Apr 2019
H5VJGBGXB_n01_CTR1-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJGBGXB_n01_CTR1-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59243634
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC363550.019.1047481
GACTAAC207500.018.6902961
GGGAATG185550.018.6481291
CAATGGA192350.018.6131538
TGACTAG230100.018.5938951
CGAACGG169550.018.5834319
GCCGAAG134150.018.4958383
GATACCA258450.018.473513
CGATACC258850.018.461192
ACACCGG215350.018.4577541
CTGCGGA166200.018.4427991
TGACGGC144450.018.3982091
CTAAGAC187550.018.286841
GAGGAGA117900.018.2573974
GGGCATC153000.018.2008171
AGGCGAA108450.018.18125210
AAAGGCG184800.018.1520889
TCGAACG173950.018.143558
GCATGCG265000.018.1072641
GCGCAAC264650.017.976411
Produced by FastQC (version 0.11.5)