FastQCFastQC Report
Thu 4 Apr 2019
H5VJGBGXB_n01_CTR1-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJGBGXB_n01_CTR1-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59773033
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC375100.019.067841
CGAACGG174300.018.721589
GACTAAC207550.018.6753581
GATACCA263350.018.6639613
CAATGGA200150.018.6484578
GGGAATG187050.018.5881231
CTAAGAC193400.018.569831
CTGCGGA170100.018.55691
TGACTAG233650.018.5223771
GCCGAAG135450.018.5042693
ACACCGG212200.018.4492741
TCGAACG177700.018.3988258
AGGCGAA107500.018.30288910
AAAGGCG185300.018.2901389
CGATACC269600.018.2508952
GAGGAGA116000.018.1127934
AAGAAGG182050.018.0976077
GACTGCG189700.018.0626681
GCGCGAT144650.018.0238931
TGACGGC148900.018.0173721
Produced by FastQC (version 0.11.5)