FastQCFastQC Report
Thu 4 Apr 2019
H5VJGBGXB_n01_CTR1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJGBGXB_n01_CTR1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35613523
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC225250.019.0825771
ACACCGG131950.018.7954541
CTGCGGA103750.018.7608951
GACTAAC122450.018.7524321
GGGAATG109750.018.606211
CAATGGA116200.018.6041728
GATACCA159350.018.5951293
CGAACGG103650.018.5674939
CTAAGAC112100.018.5347581
TGACGGC93550.018.4259991
AGGCGAA67250.018.4069410
TGACTAG142450.018.383411
GCCGAAG77650.018.3633773
CGATACC161500.018.3476542
GAGGAGA67100.018.3399734
AAAGGCG105200.018.293929
TCGAACG105800.018.2100228
AAGAAGG108900.018.1544027
GGGCATC92550.018.0235461
GCCGACA74900.018.0142233
Produced by FastQC (version 0.11.5)