FastQCFastQC Report
Thu 4 Apr 2019
H5VJGBGXB_n01_CTR1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5VJGBGXB_n01_CTR1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52821768
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC329450.019.0069921
GACTAAC181350.018.7387451
GCCGAAG119150.018.5857813
CGAACGG150150.018.5579629
ACACCGG194900.018.557051
CAATGGA178100.018.545958
TGACTAG204450.018.4712181
GATACCA231750.018.4586353
TCGAACG151300.018.437718
TGACGGC126400.018.4164731
GGGAATG168300.018.4128781
CTAAGAC168600.018.342731
CTGCGGA153050.018.3394761
GAGGAGA105300.018.1783524
CGATACC236200.018.150972
AGGCGAA98700.018.13687110
AAAGGCG163950.018.0589479
AGAGCGA178600.018.0097431
GCGCAAC234250.018.0048581
AAGAAGG161450.017.9939047
Produced by FastQC (version 0.11.5)