FastQCFastQC Report
Tue 4 Jun 2024
H5TVHDRX5_n02_Rep_2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TVHDRX5_n02_Rep_2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181714
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTTCGGGTGGATCTGGATCAACCTACACCAGGAGCGTGGCCACCAGCA9210.5068404195604082No Hit
TGGTTCGGGTGGATCTGGATCATGCGACAGCAGCGGCCACGCCCCCGAGA7770.4275950119418427No Hit
TGGTTCGGGTGGATCTGGATCACAGGACCTGGCCTTCTGGTACGGCCCCA6220.3422961356857479No Hit
TGGTTCGGGTGGATCTGGATCAAGGTGCGCCACCCCCCTGAAGGACGAGG4110.2261796009113222No Hit
TGGTTCGGGTGGATCTGGATCATGGTACAAGAACGGCGTGAAGATCGAGA2860.1573901845757619No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2340.12877378738016884No Hit
TGGTTCGGGTGGATCTGGCCTCAAGATCGGAAGAGCGTCGTGTAGGGAAA1820.10015739018457576Illumina Single End PCR Primer 1 (100% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)