FastQCFastQC Report
Tue 4 Jun 2024
H5TVHDRX5_n02_Rep_2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TVHDRX5_n02_Rep_2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362412042
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTTCGGGTGGATCTGGATCAACCTACACCAGGAGCGTGGCCACCAGC25029270.6906301971058677No Hit
ATGGTTCGGGTGGATCTGGATCATGCGACAGCAGCGGCCACGCCCCCGAG21719210.5992960355329474No Hit
ATGGTTCGGGTGGATCTGGATCACAGGACCTGGCCTTCTGGTACGGCCCC16192660.44680248235239384No Hit
ATGGTTCGGGTGGATCTGGATCAAGGTGCGCCACCCCCCTGAAGGACGAG10553110.2911909312329086No Hit
ATGGTTCGGGTGGATCTGGATCATGGTACAAGAACGGCGTGAAGATCGAG8732820.24096384744301627No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5907030.16299210057705532No Hit
ATGGTTCGGGTGGATCTGGCCTCAAGATCGGAAGAGCGTCGTGTAGGGAA4388690.12109669358061784Illumina Single End PCR Primer 1 (100% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)