FastQCFastQC Report
Tue 4 Jun 2024
H5TVHDRX5_n01_Rep_3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TVHDRX5_n01_Rep_3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798777
Sequences flagged as poor quality0
Sequence length64
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTC16227420.315307025615407No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAG13891817.39133700644861No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCA9461311.844732635015779No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCT8435210.560143819864617No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCA455185.698461523053368No Hit
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAGA401645.028186840632618No Hit
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCAG254983.1921299686896347No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCC238422.9848130329240825No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAGA79470.9948959471792503No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCC67760.8482968337846483No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTGC51340.6427325774277426No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAAA50150.6278348024542519No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGAC46020.5761307598991958No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAAGCGCATGCTCCAGACTGCCTTGGGA41560.5202954015951886No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGG39090.4893731291712205No Hit
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAGAC22360.2799279398380274No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAG22010.2755462413164125No Hit
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAAGCGCATGCTCCAGACTGCCTTGGGAA19580.24512473443777175No Hit
GAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAAAA13450.16838241461634473No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGCGGGGCTGCTAAAGCGCATGCTCCAGACTG13350.16713050075302618No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCT13150.1646266730263891No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCTAAAGCGCATGCTCCAGACTGCCTTGG12530.1568648070738141No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAA12290.15386021380184958No Hit
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCC12140.15198234300687177No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCACAGCGGGGCTGCTAAAGCGCATGC12030.15060523775722134No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCACAGCGGGGCTGCTAAAGCGCATGCTCC11900.14897774973490724No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTG11630.14559758230394715No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAAGCGGGGCTGCTAAAGCGCATGC11370.14234260625931894No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAAGCGGGGCTGCTAAAGCGCATGCTCC11160.13971358714635No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAA10650.1333288264434254No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCGCGGGGCTGCTAAAGCGCATGCTCCAGA8660.10841574056338628No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)