FastQCFastQC Report
Tue 4 Jun 2024
H5TVHDRX5_n01_Rep_2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TVHDRX5_n01_Rep_2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181714
Sequences flagged as poor quality0
Sequence length64
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTC3340718.384384252176496No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAG2766415.223923308055515No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCA1828910.064717082888496No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCT161588.891995113199863No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCA88064.84607680200755No Hit
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAGA74254.08609133033228No Hit
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCAG47722.6261047580263495No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCC44572.452755428860737No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAGA13180.7253156058421475No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCC13070.7192621372046183No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGAC9330.5134442035286219No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTGC9290.511242942205884No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAAA8220.4523592018226444No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGG7110.39127420011666686No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAAGCGCATGCTCCAGACTGCCTTGGGA6740.370912532881341No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAG4090.22507897024995321No Hit
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAGAC4080.22452865491926874No Hit
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAAGCGCATGCTCCAGACTGCCTTGGGAA3480.1915097350781998No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCT2530.13922977866317401No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGCGGGGCTGCTAAAGCGCATGCTCCAGACTG2490.13702851734043606No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCTAAAGCGCATGCTCCAGACTGCCTTGG2380.13097504870290677No Hit
GAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAAAA2310.1271228413881154No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCACAGCGGGGCTGCTAAAGCGCATGCTCC2290.12602221072674644No Hit
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCC2270.12492158006537746No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTG2200.12106937275058607No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAAGCGGGGCTGCTAAAGCGCATGCTCC2180.11996874208921712No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAA2090.11501590411305679No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAAGCGGGGCTGCTAAAGCGCATGC2080.1144655887823723No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCACAGCGGGGCTGCTAAAGCGCATGC2060.11336495812100332No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAA2010.11061338146758093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)