FastQCFastQC Report
Tue 4 Jun 2024
H5TVHDRX5_n01_Rep_2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TVHDRX5_n01_Rep_2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362412042
Sequences flagged as poor quality0
Sequence length64
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTC7357698220.302024622018493No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAG6089254216.802019509053732No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCA4139268011.42144167494302No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCT3817568110.533778289850535No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCA219517466.057123786190306No Hit
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGGGCTGCTAAAGCGCATGCTCCAGA185183145.109740255264477No Hit
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCCAG118995543.2834322872748256No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAGCGGGGCTGCTAAAGCGCATGCTCC114679153.1643305605170813No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAGA33933420.9363215364681509No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCC31258310.8625074880927937No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTGC22397010.6179985045861142No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAAA21992630.6068404868290773No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGAC21145120.583455226358069No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGG17509540.4831390232888564No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAAGCGCATGCTCCAGACTGCCTTGGGA17237300.4756271316172215No Hit
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAGAC10604970.29262189913656345No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAGCGGAACTGTAAAGCGCATGCTCCAG10432810.28787150510854165No Hit
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAAGCGCATGCTCCAGACTGCCTTGGGAA9145320.2523459195652224No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCT6011150.1658650735452107No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGCGGGGCTGCTAAAGCGCATGCTCCAGACTG5859120.16167012463675254No Hit
GAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAAAA5814290.16043313483496224No Hit
GAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCC5795010.15990114368219585No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCGGGGCTGCTAAAGCGCATGCTCCAGACTG5656910.15609056390019183No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAA5489800.1514795140278479No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCACAGCGGGGCTGCTAAAGCGCATGCTCC5351520.14766396752346325No Hit
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAAGCGGGGCTGCTAAAGCGCATGCTCC5312050.14657487567700633No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCTAAAGCGCATGCTCCAGACTGCCTTGG5273340.1455067544361564No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCAAAGCGGGGCTGCTAAAGCGCATGC5252990.14494523887812755No Hit
CGATGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCACAGCGGGGCTGCTAAAGCGCATGC5183250.1430209098846666No Hit
ATGAGGCCAGAGGCCACTTGTGTAGCGGGGCTGCTAAAGCGCATGCTCCAGACTGCCTTGGGAA4872240.13443924139805485No Hit
TGAGGCCAGATCCACCCGAACCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCGCG4266690.11773035952265627TruSeq Adapter, Index 11 (97% over 36bp)
TGAGGCCAGAGGCCACTTGTGTAGCGCCAAGTGCCCGCGGGGCTGCTAAAGCGCATGCTCCAGA3722510.10271485405002079No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)