##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H5TT7BCXX l02n02 vs7.34200000033618.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 372333 Sequences flagged as poor quality 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.40054735948734 38.0 32.0 38.0 27.0 38.0 2 33.81162024316942 38.0 32.0 38.0 27.0 38.0 3 33.60052426188385 38.0 32.0 38.0 27.0 38.0 4 33.68888602406985 38.0 32.0 38.0 27.0 38.0 5 33.61487700526142 38.0 32.0 38.0 27.0 38.0 6 35.57649738271923 38.0 38.0 40.0 27.0 40.0 7 35.68308476551904 38.0 38.0 40.0 27.0 40.0 8 35.61637029218468 38.0 38.0 40.0 27.0 40.0 9 35.634308535638795 38.0 38.0 40.0 27.0 40.0 10 35.64470245720901 38.0 38.0 40.0 27.0 40.0 11 35.59878657008645 38.0 38.0 40.0 27.0 40.0 12 35.61270421907271 38.0 38.0 40.0 27.0 40.0 13 35.46377570615551 38.0 38.0 40.0 13.0 40.0 14 35.39251154208733 38.0 38.0 40.0 13.0 40.0 15 35.490082802222744 38.0 38.0 40.0 27.0 40.0 16 35.36067982155758 38.0 38.0 40.0 13.0 40.0 17 35.35170398541091 38.0 38.0 40.0 13.0 40.0 18 35.34460012945401 38.0 38.0 40.0 13.0 40.0 19 35.32663771408927 38.0 38.0 40.0 13.0 40.0 20 35.367799792121566 38.0 38.0 40.0 13.0 40.0 21 35.33623396260874 38.0 38.0 40.0 13.0 40.0 22 35.41934504865269 38.0 38.0 40.0 13.0 40.0 23 35.24485071159419 38.0 38.0 40.0 13.0 40.0 24 35.26165556101662 38.0 38.0 40.0 13.0 40.0 25 35.296922378623435 38.0 38.0 40.0 13.0 40.0 26 35.17140570403375 38.0 38.0 40.0 13.0 40.0 27 35.13387209836356 38.0 38.0 40.0 13.0 40.0 28 35.09363661023868 38.0 38.0 40.0 13.0 40.0 29 35.00828290804199 38.0 38.0 40.0 13.0 40.0 30 35.03085410103321 38.0 38.0 40.0 13.0 40.0 31 34.980890761764336 38.0 38.0 40.0 13.0 40.0 32 34.985461938640945 38.0 38.0 40.0 13.0 40.0 33 34.87880204010926 38.0 38.0 40.0 13.0 40.0 34 34.864242492607424 38.0 38.0 40.0 13.0 40.0 35 34.797186926756424 38.0 38.0 40.0 13.0 40.0 36 34.72355122967881 38.0 38.0 40.0 13.0 40.0 37 34.70047779810009 38.0 38.0 40.0 13.0 40.0 38 34.72384934990989 38.0 38.0 40.0 13.0 40.0 39 34.68225217748628 38.0 32.0 40.0 13.0 40.0 40 34.75488608315674 38.0 38.0 40.0 13.0 40.0 41 34.527879613142 38.0 32.0 40.0 13.0 40.0 42 34.451504432859835 38.0 32.0 40.0 13.0 40.0 43 34.44274614390881 38.0 32.0 40.0 13.0 40.0 44 34.26789997126228 38.0 32.0 40.0 13.0 40.0 45 34.21378712066886 38.0 32.0 40.0 13.0 40.0 46 34.233908893383074 38.0 32.0 40.0 13.0 40.0 47 34.18272621551139 38.0 32.0 40.0 13.0 40.0 48 34.08724179699355 38.0 32.0 40.0 13.0 40.0 49 34.07235458581431 38.0 32.0 40.0 13.0 40.0 50 34.01613340746053 38.0 32.0 40.0 13.0 40.0 51 32.9859050903358 38.0 32.0 38.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.648724484854668 2201 2 0.9603757661461074 2201 3 0.6551051266197021 2201 4 0.8391346997866194 2201 5 0.6968532829404097 2201 6 0.6582755858167957 2201 7 0.8104519966823744 2201 8 0.47620440717948753 2201 9 0.9590986977122782 2201 10 0.7477340107184531 2201 11 0.8950984264024697 2201 12 0.7936065544359039 2201 13 0.9879614775038164 2201 14 0.5004244411791845 2201 15 0.7744253782570567 2201 16 1.0102192276921187 2201 17 1.0765117153393433 2201 18 0.8738366204343535 2201 19 0.98462102491915 2201 20 0.47953523818585353 2201 21 0.5827248565206631 2201 22 0.8816520945119706 2201 23 0.5992868353646585 2201 24 0.6021379533048545 2201 25 0.7407952108832916 2201 26 0.622122686232693 2201 27 0.5762255637398113 2201 28 0.8845398090492438 2201 29 0.9620674735837724 2201 30 0.634143879378648 2201 31 0.8612537929115831 2201 32 0.2891442261316115 2201 33 1.0830888176905162 2201 34 0.76376304552916 2201 35 0.944176957240046 2201 36 0.6292341799537411 2201 37 0.6728182713590769 2201 38 1.2658782584256514 2201 39 0.7820347576733866 2201 40 0.9789761016840188 2201 41 0.6104548541943444 2201 42 0.6791609312052387 2201 43 0.6680181958108591 2201 44 0.6310021029582771 2201 45 0.5170058630584577 2201 46 0.7795806586507297 2201 47 1.0150817931760727 2201 48 1.0985794498323571 2201 49 0.5744785105457098 2201 50 0.9840344306115796 2201 51 0.9110965302167671 1101 1 -0.5893179223567486 1101 2 -0.06749547942887091 1101 3 0.031934439418499494 1101 4 0.057637674130624816 1101 5 0.013400124296566673 1101 6 0.2467906035168781 1101 7 -0.25963668621241 1101 8 0.10498437871337529 1101 9 0.31738854114865234 1101 10 0.10071017947142025 1101 11 -0.018317142372396233 1101 12 0.14956071669276128 1101 13 0.09303428506558475 1101 14 0.5833958655797531 1101 15 0.03266047916577719 1101 16 0.008149960147136426 1101 17 0.3355278096424712 1101 18 -0.018003093444704632 1101 19 -0.024389595131211195 1101 20 0.03478139708516892 1101 21 -0.1030968288192895 1101 22 0.0467117638671013 1101 23 -0.07583086106977532 1101 24 0.06859903136769674 1101 25 -0.06360973784121171 1101 26 -0.38640619957439526 1101 27 -0.24908373154138275 1101 28 -0.7687684643714405 1101 29 -0.06261410738042628 1101 30 -0.0391198701195421 1101 31 -0.3561141863592425 1101 32 -0.14366479543694055 1101 33 -0.02666265123397693 1101 34 -0.4688744494527697 1101 35 -0.2215469214505177 1101 36 -0.4294607581871972 1101 37 -0.3188791119480996 1101 38 -0.2759450326951054 1101 39 -0.03830026659579744 1101 40 -0.19255048493690907 1101 41 -0.09028599467978893 1101 42 -0.3968326456715161 1101 43 -0.13581743062613327 1101 44 0.05424578019633941 1101 45 -0.09416161258111799 1101 46 -0.2107956049892863 1101 47 -0.32404086658087294 1101 48 -0.25126909584115964 1101 49 -0.2802238725789934 1101 50 -0.26860233446998194 1101 51 0.19498033007808857 2202 1 0.25193131461290363 2202 2 -0.10625742131353633 2202 3 0.11873235956323924 2202 4 0.3698255671372195 2202 5 0.18378204397061637 2202 6 0.4073321895903774 2202 7 0.1788428425106261 2202 8 0.20687375218271598 2202 9 0.014662224507603128 2202 10 0.15530270287714387 2202 11 0.34523832166037494 2202 12 0.005673417195069419 2202 13 0.0671206711331962 2202 14 0.2659384665755553 2202 15 0.03883458982173238 2202 16 0.07964314619416513 2202 17 0.15221298515783843 2202 18 0.1017798014372957 2202 19 -0.010938197406694883 2202 20 0.06965648074270803 2202 21 0.030475771220423553 2202 22 0.40641714221983705 2202 23 0.2882100005845416 2202 24 0.582050429092213 2202 25 0.08307227523510363 2202 26 0.7912309436399667 2202 27 0.7883139488463442 2202 28 0.572307941626903 2202 29 0.44261127156067204 2202 30 0.6596909057076559 2202 31 0.22107874448630582 2202 32 0.49255910524776425 2202 33 0.5003210438186443 2202 34 0.19413825178499167 2202 35 0.6492490571037379 2202 36 0.43555457596493596 2202 37 0.4015649533305279 2202 38 0.4806354146061551 2202 39 0.8063908807812155 2202 40 0.3646565665667225 2202 41 0.5240067589220843 2202 42 0.47995725805795786 2202 43 0.5484902526298541 2202 44 0.6373296730852616 2202 45 0.5660122480215151 2202 46 0.27956631041914903 2202 47 0.3436419481369768 2202 48 0.2551330046067335 2202 49 0.4081251136018835 2202 50 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-0.24169017689979455 1213 34 -0.06833881487458626 1213 35 -0.4972796993109512 1213 36 -0.5525971962172562 1213 37 -0.1245032750190731 1213 38 0.3326548780324643 1213 39 0.16277100256057508 1213 40 -0.1128302052166319 1213 41 -0.07686537274852867 1213 42 -0.36157003594082227 1213 43 0.31846020663022756 1213 44 -0.25422022188669047 1213 45 0.2772116949624035 1213 46 -0.036714367078261034 1213 47 -0.08288627649010749 1213 48 -0.05674447152929929 1213 49 0.06043971914842672 1213 50 -0.17019435946626515 1213 51 -0.21091165028197878 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 114.0 14 2124.0 15 4845.0 16 7229.0 17 5478.0 18 3784.0 19 3006.0 20 2664.0 21 2701.0 22 2864.0 23 3066.0 24 3468.0 25 3874.0 26 4182.0 27 4584.0 28 5170.0 29 5735.0 30 6324.0 31 7237.0 32 8285.0 33 10042.0 34 12897.0 35 16889.0 36 24769.0 37 41338.0 38 85774.0 39 93890.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 12.651029606906667 32.13593067546296 34.0511660640704 21.161873653559972 2 18.31856030083266 36.482675261885575 27.83507923717432 17.36368520010744 3 17.399900074675376 32.559001606326454 30.99028145633102 19.050816862667148 4 17.77233999118971 31.34421368173369 30.22090187272356 20.662544454353036 5 21.019677996422182 31.413882576673274 30.835844789333162 16.73059463757138 6 20.099965353222554 32.905575445372236 28.091284858283938 18.90317434312127 7 19.252865073631117 34.046233304416255 29.811000486129203 16.889901135823425 8 19.00825336459567 34.970577413229556 28.451681693537772 17.569487528636998 9 19.82456470183788 32.334938535872645 29.733489824913857 18.107006937375616 10 18.877456470417613 34.461624406109586 28.317124724373077 18.343794399099732 11 19.974807226941763 32.14764361626411 28.79764939072702 19.079899766067108 12 18.763579913679422 32.16448716605834 28.737984546091806 20.333948374170433 13 20.195899906803856 32.60199874843218 29.31864755474266 17.8834537900213 14 18.517563578839372 32.525722941560375 30.50521978981181 18.451493689788443 15 18.85704463477586 32.62321631442822 28.59161019839767 19.928128852398256 16 20.34173710092847 32.41050350089839 29.284806879862916 17.96295251831022 17 19.48282854326638 31.962248847134152 30.676571778488608 17.878350831110858 18 20.531462596453157 31.8622408555828 29.474580413664185 18.131716134299857 19 18.69777860141325 32.56251796107248 30.397520499123097 18.342182938391172 20 19.591975956877082 34.048467626923355 28.098922732729747 18.260633683469816 21 21.95245103832064 31.56430282650967 28.25086213379457 18.232384001375117 22 21.810163537282687 31.509597642957477 28.191582221195656 18.488656598564184 23 21.080323259018137 32.34121069043034 28.19089363553593 18.38757241501559 24 19.487125771822537 33.923665106235546 28.075674194873944 18.513534927067973 25 19.12400995882718 34.246225824732164 28.274421015596253 18.355343200844406 26 20.16367069263267 33.52133708266525 28.04747363247416 18.267518592227923 27 21.418730007815583 31.964128884627467 28.179881987360776 18.437259120196167 28 20.091692114317024 33.24255438008449 28.13395535716684 18.531798148431648 29 21.53394944847757 31.40683205625072 28.423212554353228 18.63600594091848 30 20.05462851802016 31.289732578095414 29.94040281146178 18.715236092422643 31 21.463313754085725 31.179884673128623 28.797071438738982 18.559730134046674 32 19.560447234061982 31.876572852795746 29.904950675873476 18.65802923726879 33 20.250152417325353 33.29814977452979 28.06090247171215 18.39079533643271 34 21.330368245629582 31.96386030784271 27.893310558021984 18.81246088850572 35 19.687484053253403 33.21999393016466 28.288924161973288 18.803597854608643 36 19.63269438916239 31.34667085646451 29.798325692323807 19.222309062049295 37 19.58569345182941 31.330824826163678 28.653920012461963 20.42956170954495 38 19.608522478534002 31.447655727534222 29.673706064195223 19.270115729736553 39 20.310582193896323 31.07540830385703 27.867258609900276 20.746750892346366 40 22.339411225972448 31.10763751802822 27.916139584726572 18.636811671272756 41 21.867253238364583 31.28194385133738 28.501636975503114 18.34916593479493 42 20.545318303776458 31.112471900153892 29.808531610144684 18.533678185924966 43 22.36573175087892 31.127243623315685 27.92768838647125 18.579336239334143 44 21.88847080436061 31.108980401952014 28.47021349168621 18.532335302001165 45 19.78497742612124 31.042104782546808 29.842640861809187 19.330276929522768 46 20.38632084719861 31.048013471811526 28.036999137868523 20.528666543121346 47 21.682203833665024 31.177467482065786 27.81381182973306 19.326516854536127 48 19.762954129770932 31.083197030615068 27.849263965321363 21.304584874292637 49 20.464208114778977 30.98785227202531 27.958843293503392 20.589096319692317 50 22.12132687674743 30.976840623850155 28.333239331458664 18.56859316794375 51 19.727233417397866 31.793314049520188 29.823571910091236 18.655880622990708 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 314.0 1 362.0 2 410.0 3 434.5 4 459.0 5 470.5 6 482.0 7 523.0 8 564.0 9 604.0 10 644.0 11 766.0 12 888.0 13 1082.5 14 1277.0 15 1487.0 16 1697.0 17 2069.0 18 2441.0 19 2872.0 20 3303.0 21 4113.5 22 4924.0 23 5972.5 24 7021.0 25 8793.5 26 12497.5 27 14429.0 28 16523.5 29 18618.0 30 20682.5 31 22747.0 32 24597.5 33 26448.0 34 27993.0 35 29538.0 36 30401.5 37 31265.0 38 31502.0 39 31739.0 40 30422.5 41 29106.0 42 27832.5 43 26559.0 44 25254.5 45 23950.0 46 22035.5 47 20121.0 48 18241.0 49 16361.0 50 14047.5 51 11734.0 52 10144.5 53 8555.0 54 7994.0 55 7433.0 56 8970.5 57 10508.0 58 6816.5 59 3125.0 60 2464.5 61 1804.0 62 1452.5 63 1101.0 64 912.0 65 723.0 66 628.5 67 534.0 68 430.5 69 327.0 70 279.0 71 231.0 72 192.5 73 154.0 74 130.0 75 82.5 76 59.0 77 45.5 78 32.0 79 25.5 80 19.0 81 12.5 82 6.0 83 6.0 84 6.0 85 4.0 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 1.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014771723161793341 2 0.008863033897076005 3 0.014771723161793341 4 0.011011648175155035 5 0.010474494605635277 6 0.0010743071390395156 7 0.0010743071390395156 8 0.0 9 5.371535695197578E-4 10 0.0 11 0.0010743071390395156 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 5.371535695197578E-4 19 0.0 20 5.371535695197578E-4 21 2.685767847598789E-4 22 5.371535695197578E-4 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 372333.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.8783339992896 #Duplication Level Percentage of deduplicated Percentage of total 1 93.76988072045442 77.71491493423368 2 2.7377768698263587 4.538047716659971 3 1.1771091824689122 2.9267054393486767 4 0.6694543222160293 2.2193303565555245 5 0.47722803113171375 1.977593207897877 6 0.29896735608582414 1.4866749835539288 7 0.26799576322725693 1.5547729662600192 8 0.12642833386901417 0.8382535745095883 9 0.13490164252941103 1.0062381047944766 >10 0.3357076147878665 3.752149151322093 >50 0.0028928760925599375 0.16645715212287607 >100 0.0010113839082482711 0.1291439466820764 >500 3.2295172798724945E-4 0.1870922512326471 >1k 0.0 0.0 >5k 3.2295167440784736E-4 1.502626214826599 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT 5614 1.5077900696419602 Illumina Single End PCR Primer 1 (100% over 51bp) ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTA 699 0.18773517254715538 Illumina Single End PCR Primer 1 (100% over 51bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.02282902670458971 0.0 0.0 2 0.03840648022066269 0.0 0.0 3 0.06472700512713082 0.0 0.0 4 0.08567599433840138 0.0 0.0 5 0.12059097635718563 0.0 0.0 6 0.15738599586928906 0.0 0.0 7 0.19283813145759307 0.0 0.0 8 0.24709064197908862 0.0 0.0 9 0.3048346507024626 0.0 0.0 10 0.36231008264107667 0.0 0.0 11 0.42112839850349015 0.0 0.0 12 0.4885411714782198 0.0 0.0 13 0.5597140194395877 0.0 0.0 14 0.625515331705758 0.0 0.0 15 0.703134022501363 0.0 0.0 16 0.7700096419065728 0.0 0.0 17 0.8350052238184635 0.0 0.0 18 0.9169211431702267 0.0 0.0 19 0.9913169125487131 0.0 0.0 20 1.0724271015461966 0.0 0.0 21 1.169114744059753 0.0 0.0 22 1.2625794651561908 0.0 0.0 23 1.3506726505574311 0.0 0.0 24 1.4347371841872731 0.0 0.0 25 1.521487485664714 0.0 0.0 26 1.6114607085592736 0.0 0.0 27 1.7094912349966294 0.0 0.0 28 1.794361498980751 0.0 0.0 29 1.8870204897229093 0.0 0.0 30 1.9829024018821861 0.0 0.0 31 2.0728756247767457 0.0 0.0 32 2.160700233393226 0.0 0.0 33 2.2549706848439435 0.0 0.0 34 2.337692334549986 0.0 0.0 35 2.4158481789151107 0.0 0.0 36 2.4856781429526795 0.0 0.0 37 2.5611482194702053 0.0 0.0 38 2.631515337077294 0.0 0.0 39 2.7048367993167406 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTCGGG 20 7.027549E-4 44.999462 15 CGGGGGT 20 7.027549E-4 44.999462 38 CGCCGTA 150 0.0 40.49951 45 TCGGGGG 35 6.2374256E-6 38.57097 37 CGCCGGA 65 9.094947E-12 38.076466 45 GCGGCGT 30 1.1385197E-4 37.499554 10 TCGGGGA 25 0.0021054067 35.99957 17 GGCGGTC 25 0.0021054067 35.99957 38 CGGTGGG 45 8.6797445E-7 34.99958 38 TCGCCGT 730 0.0 33.903706 45 AGCGGCG 40 1.5578724E-5 33.749596 9 GGTCGCC 855 0.0 31.315416 43 GGTGGTC 875 0.0 30.856775 40 GTGGTCG 890 0.0 30.589521 41 TGGTCGC 880 0.0 30.170094 42 GGGTCGC 30 0.005143141 29.999643 41 GGGCGCC 60 2.411798E-7 29.999643 42 TGGGCGC 45 3.4848574E-5 29.999641 41 CGGTGGT 915 0.0 29.261946 39 TCGGTGG 955 0.0 29.214312 38 >>END_MODULE