FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n02 vs7.34200000033618.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n02 vs7.34200000033618.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372333
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT56141.5077900696419602Illumina Single End PCR Primer 1 (100% over 51bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTA6990.18773517254715538Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGGG207.027549E-444.99946215
CGGGGGT207.027549E-444.99946238
CGCCGTA1500.040.4995145
TCGGGGG356.2374256E-638.5709737
CGCCGGA659.094947E-1238.07646645
GCGGCGT301.1385197E-437.49955410
TCGGGGA250.002105406735.9995717
GGCGGTC250.002105406735.9995738
CGGTGGG458.6797445E-734.9995838
TCGCCGT7300.033.90370645
AGCGGCG401.5578724E-533.7495969
GGTCGCC8550.031.31541643
GGTGGTC8750.030.85677540
GTGGTCG8900.030.58952141
TGGTCGC8800.030.17009442
GGGTCGC300.00514314129.99964341
GGGCGCC602.411798E-729.99964342
TGGGCGC453.4848574E-529.99964141
CGGTGGT9150.029.26194639
TCGGTGG9550.029.21431238
Produced by FastQC (version 0.11.2)