FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n02 vs5b.342000000335d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n02 vs5b.342000000335d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363091
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT39960.29315724335352517Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC6950.032.69730842
GGTCGCC6750.032.66613443
TCGCCGT6450.031.7436745
GTGGTCG7650.030.8818541
CTCGGTG7900.030.18938337
GGTGGTC8150.029.26332740
CGCCGTA1750.028.28525245
GTCGCCG8000.027.5620544
GATCTCG8400.027.32098434
TCGGTGG9250.027.242838
ATCTCGG8900.026.29170835
TCTCGGT9000.026.2495736
CGGTGGT9350.025.98888439
GTAGGGA9500.025.34169218
AGTGTAG9850.024.44122728
TGTAGGG10200.024.2643117
CGTGTAG9950.024.19558715
GTAGATC10050.023.95483631
GTGTAGG10450.023.89913216
AGCGTCG9700.023.89136310
Produced by FastQC (version 0.11.2)