Basic Statistics
Measure | Value |
---|---|
Filename | H5TT7BCXX l02n02 vs1.342000000335c6.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 3025371 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 22 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT | 269404 | 8.904825226393722 | Illumina Single End PCR Primer 1 (100% over 51bp) |
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGG | 6147 | 0.20318169242714365 | Illumina Single End PCR Primer 1 (98% over 50bp) |
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGACGT | 3394 | 0.11218458827033115 | Illumina Single End PCR Primer 1 (98% over 51bp) |
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTAGCCGT | 3205 | 0.10593742056759319 | Illumina Single End PCR Primer 1 (98% over 51bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCGCCGT | 29645 | 0.0 | 44.48324 | 45 |
GTGGTCG | 32175 | 0.0 | 44.16019 | 41 |
TCGGTGG | 32700 | 0.0 | 44.13238 | 38 |
GTCGCCG | 31330 | 0.0 | 44.108833 | 44 |
GGTCGCC | 31310 | 0.0 | 44.108265 | 43 |
CGGTGGT | 32510 | 0.0 | 44.10655 | 39 |
CTCGGTG | 32425 | 0.0 | 44.097267 | 37 |
GGTGGTC | 32270 | 0.0 | 44.085964 | 40 |
TGGTCGC | 31595 | 0.0 | 44.080692 | 42 |
TCTCGGT | 32420 | 0.0 | 44.04161 | 36 |
AGATCTC | 32650 | 0.0 | 44.041466 | 33 |
GATCTCG | 32655 | 0.0 | 44.03472 | 34 |
ATCTCGG | 32765 | 0.0 | 43.893757 | 35 |
GTAGATC | 33530 | 0.0 | 43.858585 | 31 |
TAGATCT | 33390 | 0.0 | 43.799896 | 32 |
GAGTGTA | 34350 | 0.0 | 43.257008 | 27 |
GTGTAGA | 34335 | 0.0 | 43.144848 | 29 |
GTAGGGA | 34575 | 0.0 | 42.97551 | 18 |
GGGAAAG | 34800 | 0.0 | 42.96273 | 21 |
TGTAGGG | 34450 | 0.0 | 42.844074 | 17 |