FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n02 vs1.342000000335c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n02 vs1.342000000335c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3025371
Sequences flagged as poor quality0
Sequence length51
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT2694048.904825226393722Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGG61470.20318169242714365Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGACGT33940.11218458827033115Illumina Single End PCR Primer 1 (98% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTAGCCGT32050.10593742056759319Illumina Single End PCR Primer 1 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT296450.044.4832445
GTGGTCG321750.044.1601941
TCGGTGG327000.044.1323838
GTCGCCG313300.044.10883344
GGTCGCC313100.044.10826543
CGGTGGT325100.044.1065539
CTCGGTG324250.044.09726737
GGTGGTC322700.044.08596440
TGGTCGC315950.044.08069242
TCTCGGT324200.044.0416136
AGATCTC326500.044.04146633
GATCTCG326550.044.0347234
ATCTCGG327650.043.89375735
GTAGATC335300.043.85858531
TAGATCT333900.043.79989632
GAGTGTA343500.043.25700827
GTGTAGA343350.043.14484829
GTAGGGA345750.042.9755118
GGGAAAG348000.042.9627321
TGTAGGG344500.042.84407417