FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n02 mp40.342000000335ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n02 mp40.342000000335ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20997269
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT431180.2053505148693385Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA78950.030.1798627
AGAGTGT82800.028.74939526
GTAGGGA87500.027.25663418
AGTGTAG90700.026.22050728
TGTAGGG91850.025.54939317
GTGTAGA94200.025.41347929
TAGATCT93400.025.34208132
TAGGGAA96350.024.86980219
AGATCTC94600.024.61629133
AAGAGTG99750.024.15738725
TCTCGGT104400.022.26246536
GGGAAAG111800.021.81533621
GTGTAGG108700.021.7130816
CTCGGTG108350.021.4716337
GGTGGTC112350.020.96751640
TCGGTGG112450.020.74878538
ATCTCGG117450.020.15283435
TGGTCGC115750.019.88511342
CGTGTAG118550.019.6812515
GTGGTCG120400.019.67774441
Produced by FastQC (version 0.11.2)