FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n02 mp39.34200000033590.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n02 mp39.34200000033590.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22712738
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT940660.4141552638876035Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC134750.037.66908643
CTCGGTG143550.036.94291337
TGGTCGC140600.036.6618642
GTGGTCG143750.036.59412441
ATCTCGG145400.036.4728735
GTCGCCG142100.035.8315244
TCGCCGT138950.035.13791345
GAGTGTA157450.034.7675627
GGTGGTC151650.034.73231540
TCTCGGT154150.034.4171636
GATCTCG156000.034.1676634
TCGGTGG156650.034.02588338
AGATCTC160850.033.2213533
AGTGTAG165750.033.01298528
AGAGTGT166800.032.764726
CGGTGGT164250.032.23229639
GTGTAGA171350.032.11789729
TAGATCT176150.030.83396332
GTAGGGA184750.029.45955518
AAGAGTG187250.029.35461225
Produced by FastQC (version 0.11.2)