FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n02 mp38.34200000033583.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n02 mp38.34200000033583.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27685011
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTTC109050.022.227021
AGCTTTA99150.021.4728471
AGCTTAC55250.021.344711
AGCTTTT147850.021.310711
AGCTTTG108850.020.9239311
AGCTTAT75050.020.5714361
AGCTTGT116100.017.7757641
AGCTTAG33600.017.5490341
AGCTTGC145000.017.1974071
AGCTTCT139850.016.8007721
AGCTTCC118800.016.6897811
GAGTGTA95650.016.65409527
GTAGGGA97550.016.51426718
AGCTTGG104500.016.2385141
AGCTTCA155450.016.070281
AGCTTAA60400.016.0594831
AGCTTGA125900.016.0345921
AGCTTCG120300.015.9578581
TGTAGGG103250.015.4282817
AGAGTGT108450.014.83369226
Produced by FastQC (version 0.11.2)