FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n02 mp37.34200000033569.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n02 mp37.34200000033569.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3758874
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT196340.5223372744071763Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT62260.16563470869201788Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA24600.039.511141
GCCGTAT25700.037.8199642
CCGTATC25600.037.35247843
CGTATCA26250.035.99944
AGCTTTG21650.030.458011
GTATCAT31100.030.45735245
AGCTTAC10450.029.9357591
GGTCGCC33300.029.93159938
ATCTCGG35550.029.49285130
TGGTCGC33850.029.44526737
GTGGTCG34500.029.15136336
CTCGGTG35450.029.13177332
TCGCCGT33850.028.84705540
GAGTGTA37100.028.5032522
GGTGGTC35400.028.41022735
CGGTGGT36250.028.36472934
TCTCGGT36400.028.3096531
TCGGTGG37250.028.0864733
GTCGCCG36200.027.78237739
AGCTTTC19750.027.5765571