FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n02 mp35.34200000033533.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n02 mp35.34200000033533.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13498832
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT162780.12058821089113489Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG22800.040.95299534
AGATCTC23200.040.3438933
TAGATCT23950.039.64417332
GTAGATC29950.032.4533431
GGTCGCC30200.030.91815843
TGGTCGC32700.028.89841342
GTCGCCG36100.026.17667844
GTGGTCG38100.025.09784141
ATCTCGG38700.024.59245335
GTAGGGA39450.024.58126818
CTCGGTG39350.024.35775837
TCGCCGT37100.024.07626245
GAGTGTA41550.023.28465727
CGGTGGT44100.022.295439
GGTGGTC44400.022.09408240
AGTGTAG44600.022.04545228
GATCAAC7000.021.540961
GATCCTA1156.7666406E-921.526981
GATCAAT8400.021.433791
TGTAGGG46350.020.63074117
Produced by FastQC (version 0.11.2)