FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n02 mp34.34200000033526.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n02 mp34.34200000033526.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10966586
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1305391.1903339836116726Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC171350.043.121531
GGTCGCC162900.042.84454343
AGATCTC171250.042.59486833
TAGATCT173750.042.44816632
GATCTCG172050.042.38372834
CTCGGTG172000.042.19983337
GTCGCCG165300.042.19526344
ATCTCGG173300.042.15591435
TGGTCGC166500.042.12087642
CGTCGTG175200.041.99469412
AGCGTCG175500.041.9357310
CGTGTAG176750.041.90647515
GCGTCGT174950.041.9003711
GTCGTGT175150.041.86537613
GTGGTCG171250.041.8591141
GTGTAGG178300.041.85765516
TGTAGGG180400.041.29556317
TCGCCGT161450.041.22255745
GTAGGGA180950.041.1323718
GAGCGTC179650.040.992049