FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n02 mp33.342000000334e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n02 mp33.342000000334e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26251026
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT6917952.6353065209717896Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT858950.043.73134632
GAGTGTA876350.043.4330227
AGATCTC861100.043.3295133
AGTGTAG883350.043.0022428
GTAGGGA882300.042.997418
GTAGATC881100.042.8464831
AGAGTGT889000.042.80233826
TGTAGGG885350.042.76803217
GTGTAGG891750.042.48884216
GTCGTGT881450.042.400813
TCGTGTA880150.042.32282614
CGTGTAG891050.042.22173715
GTGTAGA903900.042.17643429
TGTAGAT901900.042.16518830
TAGGGAA899150.042.161619
TCTCGGT885350.042.08171536
ATCTCGG891100.041.91874335
AAGAGTG910000.041.84920525
GGGAAAG921550.041.50544721
AGAGCGT917350.041.3374678