FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n01 vs7.3410000003361b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n01 vs7.3410000003361b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372333
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC59421.5958832550432005TruSeq Adapter, Index 6 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG6190.16624902976636508TruSeq Adapter, Index 6 (100% over 51bp)
TCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAA5530.14852296197221304RNA PCR Primer, Index 40 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG9650.029.14455639
ATATCTC10350.026.9560438
CAGGGAT551.3659438E-424.5450156
CAATATC12350.022.77287136
AATATCT12400.022.68104737
ACGCCAA13500.022.66626232
CGCCAAT13500.022.66626233
CACGCCA13600.022.66503531
CTGACCC500.002261992322.49959840
TCCAGGG500.002261992322.4995984
GCCAATA13550.022.41657434
CCAATAT12750.022.23489635
GTATGCC13950.022.09637645
TCTCGTA13050.021.8961641
ATCTCGT12850.021.88676640
TCACGCC14100.021.70173830
CTCGTAT14100.021.70173842
GTCACGC14450.021.33179929
TCGTATG14800.020.8273343
AGTCACA1750.020.5710611
Produced by FastQC (version 0.11.2)