FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n01 vs6a.341000000335f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n01 vs6a.341000000335f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155762
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC276982.3965141612200433TruSeq Adapter, Index 4 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGCC15500.13411065600011074TruSeq Adapter, Index 4 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA40800.037.1564061
CAATCTC34050.036.67305838
ATCTCGT34700.036.56965640
ACTGACC39600.036.5331432
ACCAATC34650.036.3626936
AATCTCG35000.036.19191739
CACTGAC40350.036.0771331
GACCAAT36800.036.07243335
CTGACCA40050.036.01030333
CCAATCT35050.035.94771237
AGTCACT40950.035.76830328
TCTCGTA35800.035.697441
GTATGCC40750.035.22607845
CCAGTCA42100.035.1653626
TCCAGTC42100.035.1119225
CAGTCAC42350.035.06402627
CTCGTAT39400.034.7199142
GTCACTG42250.034.61448729
ACTCCAG43100.034.61047423
CACACGT43950.034.50422312
Produced by FastQC (version 0.11.2)