FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n01 vs5b.341000000335d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n01 vs5b.341000000335d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363091
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC35770.26241828315204196TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG6800.028.78681239
GCACACG10450.025.4058111
ACACGTC10700.024.81221413
CACACGT11050.024.43353512
CACGTCT10800.024.16582114
ACGTCTG11000.023.93097915
AGCACAC11250.023.39918110
AGTCACC10100.022.9447428
GATCGGA11600.022.9081361
CGTCTGA11600.022.69316916
CCAGATC9750.022.38383133
GAGCACA12300.021.5846089
CAGATCA10450.021.53034634
CAGTCAC11750.021.44605827
AGAGCAC12550.021.1546388
TCACCAG11400.020.52559730
TCATCTC9750.020.30772638
TCCAGTC12450.020.24025325
CCAGTCA12350.020.22196226
GATCATC9800.020.20411536
Produced by FastQC (version 0.11.2)