Basic Statistics
Measure | Value |
---|---|
Filename | H5TT7BCXX l02n01 vs1.341000000335c9.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 3025371 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 23 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG | 274492 | 9.073002947407112 | TruSeq Adapter, Index 13 (97% over 40bp) |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 14726 | 0.48675022005565605 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTATG | 12628 | 0.4174033531755279 | TruSeq Adapter, Index 13 (97% over 40bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAAACTCGTATG | 10512 | 0.3474615179427581 | TruSeq Adapter, Index 13 (97% over 40bp) |
TCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAA | 5925 | 0.19584374941122923 | RNA PCR Primer, Index 40 (96% over 30bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3648 | 0.12058025280205305 | No Hit |
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3084 | 0.10193791108594616 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGTCAAC | 34110 | 0.0 | 43.251217 | 34 |
GTCACAG | 34590 | 0.0 | 43.19742 | 29 |
TCACAGT | 34545 | 0.0 | 43.188557 | 30 |
GTCAACA | 34150 | 0.0 | 43.09514 | 35 |
CACAGTC | 34320 | 0.0 | 43.084904 | 31 |
ACAATCT | 30820 | 0.0 | 43.06462 | 39 |
CAATCTC | 30695 | 0.0 | 43.049416 | 40 |
CAGTCAA | 34510 | 0.0 | 43.030247 | 33 |
ACAGTCA | 34585 | 0.0 | 42.94344 | 32 |
AACAATC | 31040 | 0.0 | 42.765938 | 38 |
TCAACAA | 34400 | 0.0 | 42.60465 | 36 |
CAACAAT | 32490 | 0.0 | 42.540096 | 37 |
ACGTCTG | 36805 | 0.0 | 42.070293 | 15 |
CGTCTGA | 36895 | 0.0 | 42.028652 | 16 |
ACACGTC | 36940 | 0.0 | 42.007908 | 13 |
GTCTCCC | 75 | 0.0 | 41.99926 | 42 |
CACGTCT | 36850 | 0.0 | 41.982285 | 14 |
GTCTGAA | 37085 | 0.0 | 41.958927 | 17 |
GCACACG | 37140 | 0.0 | 41.933144 | 11 |
CACACGT | 37120 | 0.0 | 41.89512 | 12 |