FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n01 vs1.341000000335c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n01 vs1.341000000335c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3025371
Sequences flagged as poor quality0
Sequence length51
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG2744929.073002947407112TruSeq Adapter, Index 13 (97% over 40bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA147260.48675022005565605No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTATG126280.4174033531755279TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAAACTCGTATG105120.3474615179427581TruSeq Adapter, Index 13 (97% over 40bp)
TCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAA59250.19584374941122923RNA PCR Primer, Index 40 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36480.12058025280205305No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30840.10193791108594616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCAAC341100.043.25121734
GTCACAG345900.043.1974229
TCACAGT345450.043.18855730
GTCAACA341500.043.0951435
CACAGTC343200.043.08490431
ACAATCT308200.043.0646239
CAATCTC306950.043.04941640
CAGTCAA345100.043.03024733
ACAGTCA345850.042.9434432
AACAATC310400.042.76593838
TCAACAA344000.042.6046536
CAACAAT324900.042.54009637
ACGTCTG368050.042.07029315
CGTCTGA368950.042.02865216
ACACGTC369400.042.00790813
GTCTCCC750.041.9992642
CACGTCT368500.041.98228514
GTCTGAA370850.041.95892717
GCACACG371400.041.93314411
CACACGT371200.041.8951212