FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n01 mp40.341000000335a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n01 mp40.341000000335a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20997269
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG418620.19936878457860402TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGA79200.029.97060236
CACCCGT82000.029.02946331
ACCCGTC82100.028.96669632
CCCGTCC84900.027.95824233
CCGTCCC87150.027.23649434
TCACCCG89500.026.7476630
AGTCACC91050.026.6134328
CAGTCAC92350.026.45806327
CGTCCCG90650.026.18489335
GTCACCC92700.026.09137329
CTCGTAT85950.025.91574744
CCAGTCA95450.025.43376426
TCCCGAT88950.024.86427337
TCGTATG100250.024.23894945
TCCAGTC103300.023.67512125
TCTCGTA87750.023.56360643
ACTCCAG105350.023.38528323
CTCCAGT106400.023.02763224
GAACTCC113750.021.83664321
CGTCTGA114200.021.65239716
Produced by FastQC (version 0.11.2)