FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n01 mp39.34100000033593.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n01 mp39.34100000033593.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22712738
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG954810.42038524813697054TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCAC170550.032.47905327
AGTCACA172800.032.1082328
CCAGTCA175950.031.3417326
TCCAGTC176350.031.25788325
TCACATG176900.031.13526230
GTCACAT178700.030.9349629
CTCGTAT169400.030.82736644
CACATGT181000.030.4797131
TCGTATG183100.030.08144645
TCTCGTA168200.029.61586443
ACTCCAG188850.029.4510923
TGTCAGA189600.029.28705835
CTCCAGT189000.029.27295924
ATCTCGT169750.029.2260242
ATGTCAG189900.029.21709434
AATCTCG171750.028.82018941
ACATGTC192800.028.7076132
CATGTCA196150.028.2058533
GTCAGAA195550.028.09685736
AGCTTAC52300.027.3079661
Produced by FastQC (version 0.11.2)