FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n01 mp38.34100000033586.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n01 mp38.34100000033586.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27685011
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC295190.10662448355176742TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTTC115300.021.6887111
AGCTTTG105600.021.6542021
AGCTTTA105700.021.2926921
AGCTTAC53350.021.2409341
AGCTTTT155750.020.6412491
AGCTTAT70850.020.509731
AGCTTAA62900.018.4099461
AGTCACG94500.017.80876228
AGCTTAG31900.017.7265031
AGCTTGT116450.016.908771
AGCTTGG103900.016.782841
AGCTTCT141550.016.4570031
ACGGCTA102150.016.34300232
AGCTTGC148450.016.2687661
AGCTTCC122050.016.2436981
AGCTTGA121450.015.7674491
AGCTTCA161100.015.7464591
AGCTTCG116200.015.5879851
CTCGTAT104550.015.51614542
CAGTCAC110450.015.42034527
Produced by FastQC (version 0.11.2)