FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n01 mp37.3410000003356c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n01 mp37.3410000003356c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3758874
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTT102030.2714376698979535TruSeq Adapter, Index 8 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC66480.17686147500554686TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT24500.033.70351444
TGCCGTC27750.031.0535343
AGCTTTG20850.030.4700531
TATGCCG29950.030.425241
CCGTCTT27600.030.40750145
ATGCCGT29300.030.25546342
AGCTTTC20900.028.5647031
AGCTTAC11450.027.939141
AGCTTGA18100.027.2581081
AGCTTTA26400.027.0511361
AGCTTAA12900.026.8943581
GTCACAC33900.026.74662424
AGCTTGC11550.026.5269361
AGCTTGG10700.025.6865771
CAGTCAC36600.025.6341322
AGCTTCT23850.025.314921
TCCAGTC39350.025.10056120
AGCTTAT14600.024.842951
AGCTTTT39750.024.8237361
AGCTTAG6900.024.8138791
Produced by FastQC (version 0.11.2)