FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n01 mp35.34100000033536.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n01 mp35.34100000033536.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13498832
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG165210.1223883666379432TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG32400.029.9987532
CGTCCGC33750.028.99879333
AGTCACG35900.027.8888128
TCCGCAC35650.026.1912535
CACGTCC38250.025.11659831
GTCCGCA38450.025.0444734
CCGCACA38150.024.82895336
GTCACGT40550.024.63531729
GATCAAT7100.023.7965831
GATCCAT1800.023.7789571
CGCACAT38000.023.3283237
GATCCTA1450.023.3042411
GATCAAC6850.023.0207351
TCACGTC43600.022.55066530
TCGTATG45250.022.52434245
GATCCAC2550.022.085721
GATCACG1900.021.341781
CTCGTAT44400.021.28328944
CAGTCAC48750.020.44530127
GATCATG2900.020.197011
Produced by FastQC (version 0.11.2)