FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n01 mp34.34100000033529.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n01 mp34.34100000033529.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10966586
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC1297041.1827199458427629TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG179900.042.4854311
AGTCACG179100.042.19667428
CACGAGT178050.042.10433231
GTGGATC152700.042.08127636
CGAGTGG179150.042.00906833
ACACGTC182350.041.9516313
CACACGT182400.041.9277912
GTCACGA180100.041.91240729
ACGTCTG183400.041.73541315
GGATCTC154750.041.5965138
ACGAGTG181150.041.57010732
TCACGAG181500.041.55192630
GATCTCG155350.041.43585639
CACGTCT185300.041.27179714
CGTCTGA185800.041.22052816
GATCGGA193000.040.9828341
AGCACAC187400.040.83313410
GTATGCC184500.040.54776845
GAGCACA192550.039.7994239
CAGTCAC190650.039.7583227
Produced by FastQC (version 0.11.2)