FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l02n01 mp33.341000000334e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l02n01 mp33.341000000334e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26251026
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC6945052.645629926997901TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGAACTCGTATGCC335400.12776643472906546TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATATCGTATGCC321940.12263901609026633TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG907750.042.2544828
GAGATCT793350.042.08286737
AGATCTC791200.042.080838
GTATGCC899800.041.96082345
AGAGATC795300.041.95422736
CGTAGAG912750.041.8258933
CTCGTAT860850.041.7919242
GTAGAGA910550.041.7268834
ACGTAGA918300.041.7127632
CGTCTGA931900.041.70573416
CACACGT934250.041.6703512
GTCACGT921250.041.6597829
TCGTATG914350.041.65490743
CAGTCAC922300.041.5390927
CACGTCT936000.041.5204914
CACGTAG923250.041.4842431
ACGTCTG938150.041.4686615
ACACGTC939450.041.44209313
TAGAGAT847850.041.42125735
GATCGGA959200.041.37751
Produced by FastQC (version 0.11.2)