FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l01n02 lacz_18hpf_21may.342000000334fe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l01n02 lacz_18hpf_21may.342000000334fe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTGTAACCATAAGGCCACGTATTTTGCAAGCTATTTAACTGGCGGCGA22.5316455696202533No Hit
TTAATCCACTGTTCACCATAAACGTGACGATGAGGGACATAAAAAGTAAAA11.2658227848101267No Hit
AATATGTTGTTAAAGTCAATTAAAAAAATCATTTTATACCAAAAGCGTATT11.2658227848101267No Hit
CTACCGCGCTTCGCTTGGTCAACCCCTCAGCGGCAAAAATTAAAATTTTTA11.2658227848101267No Hit
CATTTTAAAAGAGCGTGGATTACTATCTGAGTCCGATGCTGTTCAACCACT11.2658227848101267No Hit
GAGATATCATGAGCTGTTTGGTTTCTGGTTATAAAAGCAATTAGTACTACA11.2658227848101267No Hit
TTCCTGAATGAATGGGAAGCCTTCAAGAAGGTGATAAGCAGGAGAAACATA11.2658227848101267No Hit
GTAATGTTTCTACTACTAAGTCGTAAACCTTAATCCCGCTTCTCATATGTC11.2658227848101267No Hit
ACCGATTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGTGACTATTTTC11.2658227848101267No Hit
GCAGTTGCCTATATTCGGGAACGTGAACCTAGGACATCGGACAACGCAACG11.2658227848101267No Hit
ATTTCTTTTACAACTAACATAACTTTTTGTTACTGTTCATAGTAGGGTTTT11.2658227848101267No Hit
AGATATATTTAATTCCTAAAATTAATAGATTATTTTATCTTATCTGTATAA11.2658227848101267No Hit
GTCCGGAGCATAGAGAAAGCGGAAGAAAGGAAATAGAAAAGGTGCGGCTAC11.2658227848101267No Hit
TTCCCGAGCTCTCCGCGGGGTGCCGAGGTGCCCCTTGCCGCGGAGCCAGAT11.2658227848101267No Hit
ATTCTAAATCTAGGTAAAATTAAAATATAAACTTCTGGATGTCTAAAAAAT11.2658227848101267No Hit
GCATAATCTCGGAAACGTGCTGTTGCTTGGAAAGATTGGTGTTTTCCATAA11.2658227848101267No Hit
ATAATCACGAGTATCCTTTCCTTTATCAGCGGCAGACTTGCCAACAAGTCC11.2658227848101267No Hit
CCAAACATAAATCACCTCACTTAAGTGGCTGGAGACAAATAATCTCTTTAA11.2658227848101267No Hit
GTTAAACATATATTCAGTAAAAGCAAGAATTAAACACATACCGTCCGTTCA11.2658227848101267No Hit
ATAATGAATGAATCATAGACAAATAAATATACGACGAAAGACGGCCCTTCG11.2658227848101267No Hit
TTTTGCATCTCGGCAATCTCTTTCTGATTGTCCAGTTGCATTTTAGTAAGC11.2658227848101267No Hit
GTCTAAGGCGCTGCGTTCAGGTCGCAGTCTCCATTGGAGGCGCTAAATTTA11.2658227848101267No Hit
TGATTTATGTTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGT11.2658227848101267No Hit
AGGAGTTTAATCATGTTTCAGACTTTTATTTCTCGCCATAATTCAAACTTT11.2658227848101267No Hit
AGTAAGTAATGTGAGGATAGGTAAGTAACGTGAGGATAAATAAGTAATGTG11.2658227848101267No Hit
GTCGATGTCCATCGGAATAATTCTTCCGAGTTCTCCGAGAGTTTTCACACG11.2658227848101267No Hit
CATTTCCATGCGGTGCACTTTATGCGGACACTTCCTACAGGTAGCGTTGAC11.2658227848101267No Hit
GTTGTAGCTTGATTACTTTTTTAAAGAATAATCATTTGAATCATTTTGCCA11.2658227848101267No Hit
AAACATGAGCGCAGAATACAGCATTAAAAACATCCTGAAGTTTTTCAAAGG11.2658227848101267No Hit
CTGATGGTATTGGCTCTAATTTGTCTAGGAAATAACCGTCAGGATTGACAC11.2658227848101267No Hit
GAAAAGTGCTGGGGGATAATTTGCTAATGGCGATGGGGGCCATGCTGGAAG11.2658227848101267No Hit
GAATATATATACTAAAGGTTTCAAAATAAAAACTGTTAATCTGTTTTTCCT11.2658227848101267No Hit
ATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGTGGC11.2658227848101267No Hit
GTCTCGCTCTGTCGCCAGGCTGGAGTGCAGTGGCACGATCTTGGTTCACCA11.2658227848101267No Hit
GGTAAAATACTGACCAGCCGTTTGAGCTTGAGTAAGCATTTGGCGCATAAT11.2658227848101267No Hit
TACAAACTCTACATGAATAGGTATAAAGCTATGTAATAATCTGCAAATTTC11.2658227848101267No Hit
ATACCCGTCTATGAAGTGATAAGGGTTACAGAAACAGTAAGTAAAAGGGTC11.2658227848101267No Hit
ACAGAACGTGACGTGAAACTTCCTATAAATATATTGACTGCTGCTTGTCAG11.2658227848101267No Hit
TAAAATGTCAACAAGAGAATCTCTACCATGAACAAAATGTGACTCATATCT11.2658227848101267No Hit
CAAGTATAAATGGGTATCAGGGTTTAGGGGAAAAATCTGAATATATGACAA11.2658227848101267No Hit
GGTCTGGAAACGTACGGATTGTTCAGTAACTTGACTCATGATTTCTTACCT11.2658227848101267No Hit
GCACAGAATGTTTATAGGTCTGTTGAACACGACCAGAAAACTGGCCTAACG11.2658227848101267No Hit
TTTATACACTATTTAGTTCAGACCTTTCAATATTGGATACAGTTGTTCTTA11.2658227848101267No Hit
ATCTATAGGAGTCGTGAGAATACAGTTTTATAATTCTTTAAATATTCTTTG11.2658227848101267No Hit
AGCAGCATCAGTGACGACATTAGAAATATCCTTTGCAGTAGCGCCAATATG11.2658227848101267No Hit
AAATAGACAAGAACAGTTAAATACAACATTTTTTTTTATATCACATTTATT11.2658227848101267No Hit
CATATGTATTCAGTATCAAATAAATACTATTTAGTATTTACGATTTCGTAA11.2658227848101267No Hit
CTTTACCATTACATGATGTATCTTCAGCTGTCAGAAAACTTTGCATTGAGT11.2658227848101267No Hit
CCTTTATCTGCAAATATCAGACACTCTAATACTAGAGTTGATATAGCTATT11.2658227848101267No Hit
ATTGCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTGCTATTCAGCG11.2658227848101267No Hit
CCAGAAACAAAACTAGGGACGGCCTCATCAGGGTTAGGAACATTAGAGCCT11.2658227848101267No Hit
TAAATAGGTTACATTCGTTTACTTTAAAACATTGTAGATTACTTCTACCTT11.2658227848101267No Hit
GCGTTTGATGAATGCAATGCGACAGGCTCATGCTGATGGTTGGTTTATCGT11.2658227848101267No Hit
ATTATCGTTAAAAGATTTAGCAAATGATTAATAACATTAAAAGTAGGAACT11.2658227848101267No Hit
AAAAGGTAATTCAACGGAGCGAGCGAACGAAAAGAACCAAGAAACCACCCA11.2658227848101267No Hit
TTCTTCAATAATGTTACCAAAAGTGACATAAATAAAATAGTATTGAAAAAT11.2658227848101267No Hit
GGCGGACGCGTCAAGGAATCCAAGCGAGCGATCCAACATCGTGTTTGGTCG11.2658227848101267No Hit
CCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAGATTGCTGGAGGCCTCCA11.2658227848101267No Hit
TAAAGCTGATGGTATTGGCTCTAATTTGTCTAGGAAATAACCGTCAGGATT11.2658227848101267No Hit
GACAGCTTGGTTTTTAGTGAGTTGTTCCATTCTTTAGCTCCTAGACCTTTA11.2658227848101267No Hit
TTTTAAGACAAATTCATTTTTTTATAATATTTGTTAGAGTAAATTTAACTT11.2658227848101267No Hit
AGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGG11.2658227848101267No Hit
TCAATCTCATCTCTCTTTTTGCGTTCTGCTTCAATATCTGGTTGAACGGCG11.2658227848101267No Hit
TCTTAGATTCAAGACATGCTTTAAGCTTACCACCGCAACTTAAAGAACCCA11.2658227848101267No Hit
CAATGTTACAGTTTTGCCCCTCCTCCCTTAAATCCAGAGTTCATTGTTTTG11.2658227848101267No Hit
TGTTAGATCACCAGGATATTTAAGACCTTGAACCTTGCAACCAACATCTTC11.2658227848101267No Hit
GTCGTCTTATTACCATTTCAACTACTCCGGTTATCGCTGGCGACTCCTTCG11.2658227848101267No Hit
CGGCATTCCTGCTGAACCGCTCGCCGGATTTGAGAATCAAAAAGAGCTTAC11.2658227848101267Illumina Paired End Adapter 2 (95% over 22bp)
AGATTGCTGGCATTCAGTCGGCGACTTCACGCCAGAATACGAAAGACCAGG11.2658227848101267No Hit
ATCGGCGGAAATTCCGGGACGCCCTGTTGGGGCAAGATGGCGCTACAGGTC11.2658227848101267No Hit
TAAAGGACGGTTGTCAGCGTCATAAGAGGTTTTACCTCCAAATGAAGAAAT11.2658227848101267No Hit
GTGATTACTTCATGCAGCGTTACCATGATGTTATTTCTTCATTTGGAGGTA11.2658227848101267No Hit
TCATTATTATTGGCTTTAAAATTTCAATCAAACAAATAGGTTGGCCAGCTT11.2658227848101267No Hit
GACGCAAGCTGTGAATTCCATTAATCCTCCCAACGCTGTCTCTTATACACA11.2658227848101267No Hit
ACATAGGCATGACATAAATATCATAGGCATGACATAAATGACATATGCATG11.2658227848101267No Hit
AGATGCAAAATGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGACTT11.2658227848101267No Hit
CCAACGCGTCAGTTTTTGACAGAATCGTTAGTTGATGGCGAAAGGTCGCAA11.2658227848101267No Hit
TTCTCCTCATCCAACGCGTCAGTTTTTGACAGAATCGTTAGTTGATGGCGA11.2658227848101267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCAA50.045.016
AAATTTT50.045.043
TTCGCTT50.045.010
AATTTTT50.045.044
AAATTAA50.045.037
TAAAATT50.045.041
CAACCCC50.045.020
GTCAACC50.045.018
GGCAAAA50.045.032
CGCTTGG50.045.012
CGCTTCG50.045.07
GCGGCAA50.045.030
ACCGCGC50.045.03
AATTAAA50.045.038
AACCCCT50.045.021
TCAACCC50.045.019
GGTCAAC50.045.017
CTTGGTC50.045.014
TCAGCGG50.045.027
TCGCTTG50.045.011
Produced by FastQC (version 0.11.2)