FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l01n02 lacz_15hpf_30apr.342000000334d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l01n02 lacz_15hpf_30apr.342000000334d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72205180
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG116100.015.19362943
TCTCGGT119450.014.334290541
CTCGGTG124100.013.81531742
CTGCCGA150300.011.84118624
CGGTGGT154650.011.55176344
GTAGATC157750.011.28196636
CCGACGA155750.011.22459427
GCTGCCG162550.010.94881723
AGATCTC167850.010.93821638
ATCTCGG160700.010.78083840
GACGAGT162800.010.76615829
CGCTGCC168600.010.55596222
CTGACGC172100.010.39365918
GCCGACG170850.010.32473126
GGTGGTC171250.010.05098245
CGAGTGT176500.010.03246731
GACGCTG186400.09.92213420
TGACGCT185950.09.92201419
TGCCGAC189400.09.63426525
ACGCTGC191900.09.59085321
Produced by FastQC (version 0.11.2)